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Frequently Asked Questions

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History

  • How have been treated the cells to induce them to grow but also to become differentiated into mature hepatic cells?

Health risks

  • What should the security level of the laboratory be for handling HepaRG? cells?

Biology of the cells

  • Some tumor cell lines show genetic instability which can negatively affect historical data comparisons: what about HepaRG™?
  • Since the cells came from resected tumor tissue, will they proliferate/pile up on each other, are tumorigenic, etc?
  • What is the cellular material observed under the microscope, in between the hepatocyte colonies?
  • Are primitive biliary cells contributing to hepatocyte metabolism quantification?
  • Is there a way to change the population to be all hepatocytes?
  • Why is the 2D6 activity relatively low?
  • Some batches of plateable human hepatocytes show more fold induction (enzymatic activity) than the HepaRG™; is there something different about the HepaRG™?
  • HPR116. Since there are two different cell populations, are the imaging studies such as High Contents Screening (HCS) affected?
  • What is the role of biliary cells in the culture ?
  • With fully differentiated HepaRG™ cells, how can you explain the obtained ratio of 50% hepatocytes and 50% primitive biliary cells?
  • What is the amount of total RNA possible to extract from HepaRG™ cells ?
  • How does the size of HepaRG™ cells compare with that of primary human hepatocytes?
  • What are the donor details of the HepaRG™ cells?

Cell culture

  • How do I use medium supplements?
  • What factors influence cell attachment and growth?
  • Is Percoll separation recommended or suggested?
  • HPR116: Can I use fewer cells per well when I seed differentiated HepaRG™ in multiwell plates?
  • HPR116: Why do the User Instructions for metabolism studies indicate to either use the cells the day of thawing, or to wait until at least the fourth day after thawing to use them?
  • Is a collagen coating of the culture support beneficial?
  • After I use the cells for an experiment, can I re-use them?
  • Since proliferating HepaRG™ cells reach confluency after 5-7 days of culture in growth medium, is it really necessary to wait 14 days before proceeding to subculture ?
  • Biopredic's protocols omit the removal of DMSO (after thawing) or neutralised trypsin (when passaging) from the cells before seeding them. Do DMSO and/or trypsin affect the attachment efficiency or growth of the cells?
  • Can we use smaller volumes for culturing cells in flasks than those recommended by Biopredic?
  • Can HepaRG™ cells be cultured in sandwich format using Matrigel or collagen gel overlays? If so, what are Biopredic's recommendations for overlaying the cells?
  • When the Differentiation medium is added on the HepaRG cell line, a strange odor appears from the cell culture. is it normal?

Medium & Additives

  • Can I use a different basal medium from William's E Medium?
  • Why are there different medium supplements?
  • Are additives complete, or should I add antibiotics
  • What is the glucose content in the HepaRG™ media ?

Logistics & Distribution

  • How are the cells delivered ?
  • How must the cells be stored ?
  • How to obtain the cryopreserved differentiated HepaRG? cells (HPR116)?

Regulatory body

  • Can the HepaRG™ cell line be used as a model system recognized by the FDA, as described in the 2006 FDA Guidance on Drug Interaction Studies?

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