Send this article to a friend Print the page

Undifferentiated HepaRG cells - HPR101

The HepaRG™ cell line

HPR101 is a frozen vial of undifferentiated HepaRG™ cells at passage P12.

HPR101 HepaRG™ cells can be grown in-house up to passage 20 to increase their population or can be differentiated into fully functional hepatocytes-like cells.

HepaRG™ is patented. HPR101 is only available under a MTA for academic scientists or through a sub-license established between Biopredic International and researchers from commercial organisations. 

Read more on how to get HPR101 undifferentiated HepaRG cryopreserved cells.

Download PDF Download HPR101 Product Specifications

Key advantages

  • Flexibility - the cells can be used at your convenience
  • Reproducible experiments - identical differentiation process for each passage
  • Long living - cultures remain viable and usable for 7-8 passages
  • Long-term studies - well adapted to stable gene transfer

Storage and delivery conditions for HPR101 cells

Undifferentiated HepaRG™ cryopreserved cells are delivered in dry ice and can be stored for 5 years in liquid nitrogen.

Please follow the Instruction manual for the culture of the HepaRG cell line. The instruction manual is provided once a MTA or a sub-license agreement has been signed.

HPR101 cells, media and additives

  • HPR101 - Undifferentiated HepaRG™ cells cryopreserved
    0.5 mL vial with at least 1 x 106 viable cells

MEDIA FOR CELL CULTURE

  • MIL700 - Basal hepatic cell medium 500 ml
  • ADD710 - HepaRG™ Growth Medium Supplement with antibiotics
    65.5 mL. To be combined with 500 ml of base medium
  • ADD711 - HepaRG™ Growth Medium Supplement
    59.3 mL. To be combined with 500ml of base medium
  • ADD720 - HepaRG™ Differentiation Medium Supplement with antibiotics
    76.7 mL. To be combined with 500 ml of base medium
  • ADD721 - HepaRG™ Differentiation Medium Supplement
    70.1 mL. To be combined with 500 ml of base medium
HPR101 - P14

Microscopic observation (x100) at passage 14 after 2 weeks in the HepaRG™ Growth Medium using ADD710, and 2 weeks in HepaRG™ Differentiation Medium using ADD720.

50 % of the cells are typical hepatocyte-like cells, which are organized in well-delineated clusters with bright canaliculi-like structures.

 

Protocol for the culture of the HepaRG™ cell line

 protocol_hpr101

 

 

 

 

 

 

 

 

 

 

Every 2 weeks, confluent HepaRG™ progenitor monolayers are detached incubating with 0.05% trypsin solution for 5 min. Cells are collected in the HepaRG™ growth medium using ADD710 or ADD711.

After cell counting, cells are seeded in the same medium and placed in a CO2 incubator at 37° C. Medium is renewed every 2-3 days thereafter. Progenitors rapidly grow up in the first 5-6 days and are highly confluent after 2 weeks.

At this stage, HepaRG™ cells can be trypsinised and passaged in order to maintain the cell line, or incubated in the HepaRG™ differentiation medium using ADD720 or ADD721, which causes the cells to undergo a complete hepatocyte differentiation programme.

Within 2 weeks, two cell types are easily recognized: hepatocyte colonies and primitive biliary cells.

Cells can then be maintained in differentiation medium for 4 further weeks.

 

BIOPREDIC International   |   customersinquiries@biopredic.com | Phone: +33 (0)2 99 14 36 14 | Parc d'Affaires de la Bretèche 35760 Saint Grégoire FRANCE

Home  |  Search  |  Site Map  |  Legal disclaimer