Differentiated HepaRG No-Spin cells - HPR116NS
Save time on washing and counting your cells
HepaRG™ No-Spin cells not only present all the advantages of differentiated HepaRG™ cryopreserved cells HPR116 but also allow direct thawing and seeding of cryopreserved differentiated cells without the need for post-thaw washing, counting and centrifugation steps.
HepaRG™ HPR116NS cells are cryopreserved after differentiation, using a proprietary process developed by Biopredic International.
A few days after thawing and seeding, the cells will form a co-culture of hepatocytes and biliary epithelial-like cells.
HPR116-NS are provided in 0.5 mL vials with ≥ 8 x 106 viable cells
Cell seeding:
- One vial will seed one 96-well plate (72 000 cells/well)
- One vial will seed 16 wells of one 24-well plate (480 000 cells/well)
Please carefully respect recommended cell seeding density.
The starting volume of cell suspension should be 8 mL for one vial. According to the cell culture support used, a volume of plating medium should be added to achieve the total volume of cell suspension needed, indicated on the certificate of analysis (COA).
 Download Product Specification Sheet |
Download User Guide |
Protocol - HPR116NS - Comparison with HPR116
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HPR116 |
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HPR116NS |
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Classical freezing process |
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Improved freezing process |
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1 mL / 8 million cells / 10% DMSO |
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0.5 mL / 8 million cells / 10% DMSO | |
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Classical thawing process |
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Improved thawing process |
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| 1. | Thaw and dilute cells with 9 mL of thawing medium (10 ml / 8 million cells / 1% DMSO) | 1. | Thaw and dilute cells with 7.5 mL of thawing/plating medium (8 ml / 8 million cells / 0.5% DMSO) | |
| 2. | Spin-down wash cells and re-suspend cells with 5 mL thawing /plating medium with 0.5% DMSO | 2 steps saved | ||
| 3. | Count cells for viability, concentration and yield | |||
| 4. | Adjust cell concentration to 0.96 million cells/mL and plate cells. | 2. | Add a volume of plating medium to achieve the total volume of cell suspension needed for plating, indicated on certificate of analysis (COA). | |
Storage and delivery conditions for HPR116NS
Differentiated HepaRG™ No-Spin cryopreserved cells are delivered in dry ice or in a nitrogen vapour dewar. If you receive them in dry ice, ensure shipment time did not exceed 48 hours.
HPR116NS cells can be stored for 5 years in liquid nitrogen.
Please follow description and user guide for the thawing, culture and use of HPR116NS.
Download the user guide for HPR116NS.
Quality controls include
- Number of viable cells per vial
- Post-thaw viability
- Cell confluence 6 hours after thawing and seeding cells ≥85%
- Cell morphology
- Mycoplasma detection (negative)
- Microbial sterility
- Enzymatic activities
- Inducibility of CYP1A2, CYP3A4/5, CYP2B6 at day 3 (48 hours induction)
- Uptake transporter activities
HPR116NS - Quick links
Key advantages of HPR116NS
- Very easy to use
- Direct cell thawing and plating thanks to an improved freezing method at Biopredic International
- Saves removing the cryoprotective agent
- Time-Saving - no more post-thaw centrifuation, washing and steps required
- Cryo-pleatable after thawing
- Cell viability > 85% after thawing
- Maintains hepatocyte functions
- Robust - hepatocyte differentiation level reproducible between batches
- Reproducible - cells from every tube have the same behavior
- Long living - HepaRG™ cell cultures remain viable and usable for 2 weeks
- Suitable for both acute and chronic studies
- Respond and function like primary human hepatocytes across a range of applications
HPR116NS - cells, media, supplements and plates
- HPR116NS - Differentiated HepaRG™-NS cryopreserved cells
≥ 8M viable cells / 2 mL vial, shipped on dry ice or in a nitrogen vapour dewar.
The following supplements are available from Biopredic International:
- MHTAP - HepaRG™-NS Thawing/ Plating Medium Supplement with corticoids
To be combined with 100 mL of Basal medium without phenol red - MHMET - HepaRG™-NS Maintenance/ Metabolism Medium Supplement with corticoids
- MHPIT - HepaRG™-NS Pre-induction/ Tox Medium Supplement with corticoids
To be combined with 100 mL of Basal medium without phenol red - MHIND - HepaRG™-NS Serum-free Induction Medium Supplement with corticoids
To be combined with 100 mL of Basal medium without phenol red
The following plates are provided by Biopredic International with HPR116NS cells, with a proprietary coating process to ensure proper seeding and culture of the HPR116NS:
- PLA135C - 6-well plate coated with collagen I
1 plate - PLA138C - 12-well plate coated with collagen I
1 plate - PLA137C - 24-well plate coated with collagen I
1 plate - PLA139C - 48-well plate coated with collagen I
1 plate - PLA136C - 96-well plate coated with collagen I
1 plate
Media per application with HPR116NS
| Media | Catalogue reference | Renewal of the medium | Use |
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HepaRG™-NS Thawing/Plating Medium |
Base medium |
/ |
- Thawing |
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HepaRG™-NS Pre-induction/Tox Medium |
Base medium |
Every 2-3 days | - Use for induction studies, up to 3 days - Use for toxicity studies, up to the use of cells |
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HepaRG™-NS Serum-free Induction Medium |
Base medium |
Daily following use of 650 | - Use for induction studies, from the 3rd day |
BIOPREDIC International | customersinquiries@biopredic.com | Phone: +33 (0)2 99 14 36 14 | Parc d'Affaires de la Bretèche 35760 Saint Grégoire FRANCE
