HepaRG™ No-Spin cells not only present all the advantages of differentiated HepaRG™ cryopreserved cells HPR116 but also allow direct thawing and seeding of cryopreserved differentiated cells without the need for post-thaw washing, counting and centrifugation steps.
HepaRG™ HPR116NS cells are cryopreserved after differentiation, using a proprietary process developed by Biopredic International.
A few days after thawing and seeding, the cells will form a co-culture of hepatocytes and biliary epithelial-like cells.
HPR116-NS are provided in 0.5 mL vials with ≥ 8 x 106 viable cells
Cell seeding:
- One vial will seed one 96-well plate (72 000 cells/well)
- One vial will seed 16 wells of one 24-well plate (480 000 cells/well)
Please carefully respect recommended cell seeding density.
The starting volume of cell suspension should be 8 mL for one vial. According to the cell culture support used, a volume of plating medium should be added to achieve the total volume of cell suspension needed, indicated on the certificate of analysis (COA).
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HPR116 |
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HPR116NS |
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Classical freezing process |
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Improved freezing process |
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1 mL / 8 million cells / 10% DMSO |
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0.5 mL / 8 million cells / 10% DMSO | |
Classical thawing process |
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Improved thawing process |
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1. | Thaw and dilute cells with 9 mL of thawing medium (10 ml / 8 million cells / 1% DMSO) | 1. | Thaw and dilute cells with 7.5 mL of thawing/plating medium (8 ml / 8 million cells / 0.5% DMSO) | |
2. | Spin-down wash cells and re-suspend cells with 5 mL thawing /plating medium with 0.5% DMSO | 2 steps saved | ||
3. | Count cells for viability, concentration and yield | |||
4. | Adjust cell concentration to 0.96 million cells/mL and plate cells. | 2. | Add a volume of plating medium to achieve the total volume of cell suspension needed for plating, indicated on certificate of analysis (COA). |
Differentiated HepaRG™ No-Spin cryopreserved cells are delivered in dry ice or in a nitrogen vapour dewar. If you receive them in dry ice, ensure shipment time did not exceed 48 hours.
HPR116NS cells can be stored for 5 years in liquid nitrogen.
Please follow description and user guide for the thawing, culture and use of HPR116NS.
Download the user guide for HPR116NS.
The following supplements are available from Biopredic International:
The following plates are provided by Biopredic International with HPR116NS cells, with a proprietary coating process to ensure proper seeding and culture of the HPR116NS:
Media | Catalogue reference | Renewal of the medium | Use |
HepaRG™-NS Thawing/Plating Medium |
Base medium |
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- Thawing |
HepaRG™-NS Pre-induction/Tox Medium |
Base medium |
Every 2-3 days | - Use for induction studies, up to 3 days - Use for toxicity studies, up to the use of cells |
HepaRG™-NS Serum-free Induction Medium |
Base medium |
Daily following use of 650 | - Use for induction studies, from the 3rd day |
BIOPREDIC International | customersinquiries@wepredic.com | Phone: +33 (0)2 99 14 36 14 | Parc d'Affaires de la Bretèche 35760 Saint Grégoire FRANCE